ABSTRACT
Human enteroviruses [EV] cause a wide spectrum of both common and uncommon illnesses among all age groups. Enterically transmitted. The objective of this study was to identify non-poliovirus EV as a cause of viral aseptic meningitis [VAM] by two methods [cell culture and Real time PCR]. From October 2010 to August 2011 cerebrospinal fluid [CSF] samples were collected from 85 patients Embaba fever hospital admitted with symptoms of aseptic meningitis of any age and both sexes. The 85 CSF samples were inoculated into RD [human rhabdomyosarcoma] cell line in three blind passages to amplify isolates producing EV-like CPE. A total of 14 [16.5%] out of 85 CSF samples showed EV-like CPE. By Real time PCR 11 out of the 14 culture positive samples and 5 out of the 14 source of virus isolation original CSF were non polio EV positive. The frequency of non-polio EV meningitis hospital admissions was in the summer season [50%], spring [25%], late autumn [16.6%] and least frequency in winter [8.4%]. non-polio EV meningitis was detected in 6 out of 41 male patients [14.5%] and in 6 out of 44 female patients [13.5%]. Also non-polio EV meningitis was detected in all ages with marked increase of incidence in young children [41.6%] and old age [50%] and less in adult [8.4%]. Our data showed that the non-polioviruses EV was associated with the majority of VAM during 2010 - 2011 at the Embaba fever hospital which serves Embaba, Shoubra Elkheema, Qualyba and neighbors localities in Egypt. Rapid detection of non-polio EV meningitis is essential for making decisions about patient management and treatment